Anticoagulant therapeutic drug monitoring assay kits are diagnostic tools used to measure the anticoagulant drug levels in a patient's blood. Anticoagulants are medications that help stop blood clots from developing or growing. They are used to treat and prevent conditions like deep vein thrombosis (DVT), pulmonary embolism (PE), atrial fibrillation, and other clotting diseases. Therapeutic drug monitoring (TDM) assay refers to estimating drug levels and metabolites in blood or serum/plasma at a particular time to determine if a patient's drug concentrations are within the therapeutic range and ensure concentrations are neither subtherapeutic nor potentially harmful. Depending on the anticoagulant being monitored, the assay kits may use different techniques for detection, such as HPLC, radioimmunoassay, homogenous enzyme immunoassay, ELISA, or other assays that produce a measurable signal indicating the drug concentration.
HPLC is used to separate the constituents of a compound, tell how much of each compound is found within the mixture, and help to identify each compound. Separation using HPLC is based on the affinity of the different mixtures within the analyte to the mobile phase and the stationary phase. The unique intermolecular interactions between the molecules of a compound of the sample and the packing material result, in consequence, in these molecules being taken up transitorily onto the stationary phase. Volatile compounds like VOCs and SVOCs are usually best analyzed by GC or GC-MS. Still, HPLC is appropriate to a much greater variety of mixtures, including non-volatile sensitivity and thermally unstable molecules.
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